Journal: The Journal of biological chemistry

Article Title: Pancreatic stone protein (lithostathine), a physiologically relevant pancreatic calcium carbonate crystal inhibitor?

doi: 10.1074/jbc.272.5.3073

Figure Lengend Snippet: FIG. 2. Analysis of the fractions separated by size exclusion column chromatography. The arrow marks recombinant rat litho- stathine. Lane S represents the selectively concentrated media proteins as loaded onto the column. Proteins were separated by SDS-PAGE (15% acrylamide) and visualized by silver staining. Molecular mass markers are shown at the right (in kDa).

Article Snippet: The fractions were evaluated by SDS-PAGE, and those containing relevant amounts of rat lithostathine were concentrated to a final volume of 9 ml in a Centriprep 10 device and then loaded onto a size exclusion chromatography column (Econo column 25/1200; BioRad) with a P10 matrix (Bio-Rad) and a bed volume of 540 ml.

Techniques: Column Chromatography, Recombinant, SDS Page, Silver Staining

Journal: The Journal of biological chemistry

Article Title: Pancreatic stone protein (lithostathine), a physiologically relevant pancreatic calcium carbonate crystal inhibitor?

doi: 10.1074/jbc.272.5.3073

Figure Lengend Snippet: FIG. 3. Analysis of the fractions eluted from the anion ex- change column. The size exclusion chromatography fractions that were most enriched with lithostathine were pooled and loaded onto a DEAE-Sephacel column (lane S). Proteins were eluted with a gradient of 10–300 mM NaCl. Lane L contains lithostathine purified to apparent homogeneity. Proteins were separated by SDS-PAGE (15% acrylamide) and visualized by silver staining. Molecular mass markers are shown at the right (kDa).

Article Snippet: The fractions were evaluated by SDS-PAGE, and those containing relevant amounts of rat lithostathine were concentrated to a final volume of 9 ml in a Centriprep 10 device and then loaded onto a size exclusion chromatography column (Econo column 25/1200; BioRad) with a P10 matrix (Bio-Rad) and a bed volume of 540 ml.

Techniques: Size-exclusion Chromatography, Purification, SDS Page, Silver Staining

Journal: The Journal of biological chemistry

Article Title: Pancreatic stone protein (lithostathine), a physiologically relevant pancreatic calcium carbonate crystal inhibitor?

doi: 10.1074/jbc.272.5.3073

Figure Lengend Snippet: FIG. 4. Immunodetection of rat lithostathine. Proteins contained in the fractions of the anion exchange chromatography were separated by SDS-PAGE (see Fig. 3) and transblotted onto a polyvinylidene diflu- oride membrane. Baculovirus expressed rat lithostathine was detected using anti-rat lithostathine antibodies. L and S as in Fig. 3. Molecular mass markers are shown at the right (in kDa).

Article Snippet: The fractions were evaluated by SDS-PAGE, and those containing relevant amounts of rat lithostathine were concentrated to a final volume of 9 ml in a Centriprep 10 device and then loaded onto a size exclusion chromatography column (Econo column 25/1200; BioRad) with a P10 matrix (Bio-Rad) and a bed volume of 540 ml.

Techniques: Immunodetection, Chromatography, SDS Page, Membrane